Direct measurement of inositol in bovine myelin basic protein.

نویسندگان

  • R Smith
  • P E Braun
  • M A Ferguson
  • M G Low
  • W R Sherman
چکیده

Myelin basic protein has been isolated from bovine central-nervous-system myelin by four methods, none of which exposes the protein to acid. After purification the inositol content of both hydrolysed and unhydrolysed protein was quantified by g.c.-m.s. Basic protein prepared by all methods contained less than 4 mol % of inositol. It is concluded, contrary to a previous proposal, that covalent binding to phosphoinositides does not represent a general mechanism for attachment of this cytoplasmically-oriented protein to its membrane.

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عنوان ژورنال:
  • The Biochemical journal

دوره 248 1  شماره 

صفحات  -

تاریخ انتشار 1987